The Harvard Glee Club is a 60-voice, Tenor-Bass choral ensemble at Harvard University. Founded in 1858 in the tradition of English and American glee clubs, it is the oldest collegiate chorus in the United States. The Glee Club is part of the Harvard Choruses of Harvard University, which also include the treble voice Radcliffe Choral Society and the mixed-voice Harvard-Radcliffe Collegium Musicum. All three groups are led by Harvard's current Director of Choral Activities Andrew Gregory Clark.

A glee club in the United States is a musical group or choir group, historically of male voices but also of female or mixed voices, which traditionally specializes in the singing of short songs by trios or quartets. In the late 19th century it was very popular in most schools and was made a tradition to have in American high schools from then on.