Healesville Sanctuary, formally known as the Sir Colin MacKenzie Sanctuary, is a zoo specialising in native Australian animals. It is located at Healesville in rural Victoria, Australia, and has a history of breeding native animals. It is one of only two places to have successfully bred a platypus, the other being Sydney's Taronga Zoo. It also assists with a breeding population of the endangered helmeted honeyeater.

Healesville is a town in Victoria, Australia, 64 km north-east from Melbourne's central business district, located within the Shire of Yarra Ranges local government area. Healesville recorded a population of 7,589 in the 2021 census.