Henley Brook is an outer rural suburb of Perth, Western Australia, part of the Swan Valley wine region. The Ellen brook and Swan River meet in the northeastern corner of the suburb. This is also the farthest upstream Captain Stirling's 1827 exploration reached before deciding on the settlement site of the new colony in 1829. The All Saints Church, the oldest church in Western Australia, is also at this site.

The Swan Valley is a region in the upper reaches of the Swan River between Guildford and Bells Rapids, Western Australia. It is bordered to the east by the Darling Scarp. Both Ellenbrook and Jane Brook lie within the region and discharge into the Swan River. There are seven suburbs within the region: Baskerville, Belhus, Caversham, Henley Brook, Herne Hill, Millendon and West Swan. It is part of the City of Swan local government area.