The Henley branch line is a branch railway line between Twyford in Berkshire and Henley-on-Thames in Oxfordshire. It was built by the Great Western Railway in 1857. Train services are provided by the present day Great Western Railway train operating company.

Wargrave is a railway station in the village of Wargrave in Berkshire, England. The station is on the Henley-on-Thames branch line that links the towns of Henley-on-Thames and Twyford. It is 1 mile 67 chains (3.0 km) down the line from Twyford and 32 miles 68 chains (52.9 km) from London Paddington.