Her Majesty's Theatre is a 1,700-seat theatre in Melbourne's East End Theatre District, Australia. Built in 1886, it is located at 219 Exhibition Street, Melbourne. It is classified by the National Trust of Australia and is listed on the Victorian Heritage Register.

The East End Theatre District is a precinct within the Melbourne central business district, and is bounded by Spring, Flinders, Swanston and Lonsdale Streets. The district is home to seven major theatres, including the Princess Theatre, Her Majesty's Theatre and the Regent Theatre. These theatres mostly house commercial productions of musicals, plays and other events, in contrast with the Southbank Arts Precinct over the Yarra River which focuses on publicly funded companies.