High Street is the main street running through the City of Fremantle, Western Australia. The street passes by historic landmarks, including the Round House, the Fremantle Town Hall, and the Fremantle War Memorial, through the Fremantle West End Heritage area and through two town squares. Trams operated along High Street for 47 years, between 1905 and 1952. Running east–west, High Street continues as Leach Highway, a major arterial road, at Stirling Highway, linking Fremantle with Perth Airport although the stretch of road between Stirling Highway and Carrington Street is known locally—and signed—as High Street.

The P&O Hotel is a heritage listed building located at 25 High Street on the corner of Mouat Street in Fremantle, Western Australia. It was one of many commercial buildings constructed in Fremantle during the gold boom period in the late nineteenth and early twentieth century.