Highland County is a county located in the U. S. state of Ohio. As of the 2010 census, the population was 43,589, its county seat is Hillsboro. The county is named for the topography, hilly and divides the watersheds of the Little Miami and Scioto Rivers. According to the U. S. Census Bureau, the county has a total area of 558 square miles, of which 553 square miles is land and 4.7 square miles is water. Fayette County Ross County Pike County Adams County Brown County Clinton County As of the census of 2010, there were 43,589 people living in Highland County; the population density is 78.8 people per square mile. The county is made up of 96.4% White, 1.5% Black or African American, 0.3% Native American, 0.3% Asian, 1.5% from two or more races, 0.8% Hispanic or Latino. This county is made up of 6.2% of people under the age of 5, 24.7% of people under the age of 18, 16.5% of people over the age of 65. The median age is 39.2. The county is 51% female. There are 16,963 households in Highland County as of the 2010 census.

The average household size is 2.58 persons. According to the 2010 census, 41.9% of the houses in Highland County had children under the age of 18 living with them, 53.07% of the houses were married couples living together, 11.09% of the houses had a female householder with no husband present, 29.91% of the houses were non-families. Individuals made up 24.33% of all households and 11.02% had someone living alone, 65 years of age or older. According to the census, 81.5% of people over the age of 25 is at least a high school graduate and 11.1% of people over the age of 25 have earned at least a bachelor's degree. The median household income is $39,641; the per capita income of Highland County is $19,557. As of the 2010 United States Census, there were 43,589 people, 16,693 households, 11,819 families residing in the county; the population density was 78.8 inhabitants per square mile. There were 19,380 housing units at an average density of 35.0 per square mile. The racial makeup of the county was 96.5% white, 1.4% black or African American, 0.3% American Indian, 0.2% Asian, 0.1% from other races, 1.5% from two or more races.

Those of Hispanic or Latino origin made up 0.7% of the population. In terms of ancestry, 23.8% were German, 14.9% were Irish, 14.3% were American, 10.7% were English. Of the 16,693 households, 34.0% had children under the age of 18 living with them, 53.1% were married couples living together, 11.9% had a female householder with no husband present, 29.2% were non-families, 24.3% of all households were made up of individuals. The average household size was 2.58 and the average family size was 3.04. The median age was 39.2 years. The median income for a household in the county was $39,844 and the median income for a family was $48,604. Males had a median income of $38,892 versus $29,167 for females; the per capita income for the county was $18,966. About 12.4% of families and 16.2% of the population were below the poverty line, including 22.2% of those under age 18 and 12.7% of those age 65 or over. Highland County is a Republican stronghold county in presidential elections; the last time it voted for Democrat was for Lyndon B. Johnson in 1964.

Highland County is home to five school districts: Greenfield Exempted Village Schools in Greenfield, Hillsboro City Schools in Hillsboro, Fairfield Local School District in Leesburg, Bright Local School District in Mowrystown, Lynchburg-Clay Local School District in Lynchburg. The Highland County District Library is the public library system serving Ohio; the main library is in Hillsboro, with three branches located in Greenfield and Lynchburg. The library is a member of the SEO Consortium which allows patrons access to over 6.9 million items owned by member libraries. There are several recreational areas in Highland County. There is Rocky Fork State Park, located 5 miles east of Hillsboro, it provides opportunities to go camping, boating, hunting, putt-putt, disc golf amongst other activities. There is Paint Creek State Park located 13 miles east of Hillsboro. Paint Creek offers many of the same activities as Rocky Fork but offers horse riding, mountain biking, winter recreational activities.

Fort Hill State Memorial is a memorial built by the Hopewell people, located 10 miles southeast of Hillsboro and is believed to be around 2000 years old. Fallsville Wildlife Area is located 4 miles north of Hillsboro, it offers fishing, hunting and wildlife watching. Habitat for Humanity of Highland County was established in 2000, has since built three homes: one on Johnson St. in Hillsboro and two on 2nd St. in Greenfield. Highland County Habitat is a locally run affiliate of Habitat for Humanity International, a nonprofit, ecumenical Christian housing organization. Habitat for Humanity works in partnership with people in need to build and renovate decent, affordable housing; the houses are sold to those in need at no profit and with no interest charged. Highlands Sanctuary is a chain of nature preserves centered in Highland County and owned by an organization of the same name. Hillsboro Greenfield Highland Leesburg Lynchburg Mowrystown Sardinia Sinking Spring Buford Highland Holiday Rocky Fork Point Fort Hill State Memorial National Register of Historic Places listings in Highland County, Ohio Highland County Airport Highland County Courthouse Rocky Fork State Park Paint Creek State Park Official website

H3K9ac is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark; the H3K9 histone has two jobs. Genes get silences them if methylated. H3K9ac is an important acetylation and connected with active promoters. H3K9ac and H3K14ac have been shown to be part of the active promoter state, they are present over bivalent promoters and active enhancers. This is a mark for liver cancer through a defect in the H3K9ac/H3K9me3 transition. Proteins are acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. In histone acetylation and deacetylation, histone proteins are acetylated and deacetylated on lysine residues in the N-terminal tail as part of gene regulation; these reactions are catalyzed by enzymes with histone acetyltransferase or histone deacetylase activity, although HATs and HDACs can modify the acetylation status of non-histone proteins as well. The regulation of transcription factors, effector proteins, molecular chaperones, cytoskeletal proteins by acetylation and deacetylation is a significant post-translational regulatory mechanism These regulatory mechanisms are analogous to phosphorylation and dephosphorylation by the action of kinases and phosphatases.

Not only can the acetylation state of a protein modify its activity but there has been recent suggestion that this post-translational modification may crosstalk with phosphorylation, ubiquitination and others for dynamic control of cellular signaling. In the field of epigenetics, histone acetylation have been shown to be important mechanisms in the regulation of gene transcription. Histones, are not the only proteins regulated by posttranslational acetylation. H3K9ac indicates acetylation of lysine 9 on histone H3 protein subunit: The genomic DNA of eukaryotic cells is wrapped around special protein molecules known as histones; the complexes formed by the looping of the DNA are known as chromatin. The basic structural unit of chromatin is the nucleosome: this consists of the core octamer of histones as well as a linker histone and about 180 base pairs of DNA; these core histones are rich in arginine residues. The carboxyl terminal end of these histones contribute to histone-histone interactions, as well as histone-DNA interactions.

The amino terminal charged tails are the site of the post-translational modifications, such as the one seen in H3K36me3. The post-translational modification of histone tails by either histone modifying complexes or chromatin remodelling complexes are interpreted by the cell and lead to complex, combinatorial transcriptional output, it is thought that a Histone code dictates the expression of genes by a complex interaction between the histones in a particular region. The current understanding and interpretation of histones comes from two large scale projects: ENCODE and the Epigenomic roadmap; the purpose of the epigenomic study was to investigate epigenetic changes across the entire genome. This led to chromatin states which define genomic regions by grouping the interactions of different proteins and/or histone modifications together. Chromatin states were investigated in Drosophila cells by looking at the binding location of proteins in the genome. Use of ChIP-sequencing revealed regions in the genome characterised by different banding.

Different developmental stages were profiled in Drosophila as well, an emphasis was placed on histone modification relevance. A look in to the data obtained led to the definition of chromatin states based on histone modifications; the human genome was annotated with chromatin states. These annotated states can be used as new ways to annotate a genome independently of the underlying genome sequence; this independence from the DNA sequence enforces the epigenetic nature of histone modifications. Chromatin states are useful in identifying regulatory elements that have no defined sequence, such as enhancers; this additional level of annotation allows for a deeper understanding of cell specific gene regulation. H3K9ac and H3K14ac have been shown to be part of the active promoter state, they are present over bivalent promoters and active enhancers. The H3K9 histone has two jobs. Genes get silences them if methylated. H3K9ac is an important acetylation and connected with active promoters; this is a mark for liver cancer through a defect in the H3K9ac/H3K9me3 transition.

Lower acetylation at this mark shows a poor prognosis in oral cancer. The histone mark acetylation can be detected in a variety of ways: 1. Chromatin Immunoprecipitation Sequencing measures the amount of DNA enrichment once bound to a targeted protein and immunoprecipitated, it results in good optimization and is used in vivo to reveal DNA-protein binding occurring in cells. ChIP-Seq can be used to identify and quantify various DNA fragments for different histone modifications along a genomic region.2. Micrococcal Nuclease sequencing is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease enzyme is employed to identify nucleosome positioning. Well positioned nucleosomes are seen to have enrichment of sequences.3. Assay for transposase accessible chromatin sequencing is used to look in to regions that are nucleosome free, it uses hyperactive Tn5 transposon to highlight nucleosome localisation. Histone acetylation

Cape Brown is a prominent ice-covered cape 5.5 nautical miles north-northeast of the summit of Mount Nicholas, marking the eastern side of the entrance to Schokalsky Bay on the northeast coast of Alexander Island in Antarctica. It was first seen from a distance by the French Antarctic Expedition under Jean-Baptiste Charcot in 1909, but charted as part of a small island, it was photographed from the air in 1937 by the British Graham Land Expedition under John Rymill, roughly mapped from the photos. It was surveyed from the ground in 1948 by Colin C. Brown, Falkland Islands Dependencies Survey surveyor at Stonington Island, 1948–49, for whom the cape is named. Cape Vostok Kosar Point Cape Brown on USGS website Cape Brown on AADC website Cape Brown on SCAR website This article incorporates public domain material from the United States Geological Survey document: "Brown, Cape"

Iryna Vladimirovna Shymanovich is a Belarusian tennis player. She has won five doubles titles on the ITF Women's Circuit. On 29 September 2014, she reached her best singles ranking of world No. 367. On 18 June 2018, she peaked at No. 372 in the doubles rankings. Shymanovich was born in Minsk. At the 2013 Wimbledon Championships, she was runner-up in girls' doubles with Anhelina Kalinina. On 4 February 2014, she made her debut for the Belarus Fed Cup team, winning her first international rubber with Ilona Kremen in doubles against Turkey. Iryna Shymanovich at the Women's Tennis Association Iryna Shymanovich at the International Tennis Federation Iryna Shymanovich at the Fed Cup

Phra Rod Meree is a 1981 Thai fantasy film. Directed by Sompote Sands, it is one of the most successful Thai feature films made in Thailand; the story is based on The Twelve Sisters, a Thai folklore myth that originated in one of the Jataka tales. This same story was adapted into a Thai fantasy lakhon. Twelve girls are abandoned by their parents; the twelve daughters are rescued by an ogress in disguise who promises to take care about them as her own daughters. Phra Rodasan, the only surviving son of the twelve sisters, goes on a quest to the ogre kingdom in order to heal his mother and his aunts' blindness. There he falls in love with Meree; the Twelve Sisters Nang Sib Song Ka Kee Phra Rod Meree on IMDb

The Illinois State Bar Association High School Mock Trial Invitational is a mock trial tournament, held every year since 1983. The event is administered by the Illinois State Bar Association; the winning team represents the state of Illinois at the National High School Mock Trial Championship. Held in Springfield, the invitational moved to Champaign, Illinois in 2013, before moving back to Springfield for the 2019 competition. In 2019, 40 high schools participated; the defending champion is York Community High School. Each school must submit a roster of no more than ten members; the tournament consists of two preliminary rounds, with each school having a chance to represent both the plaintiff/prosecution and defense. After the first two rounds, the eight highest scoring teams advance to a final round; the team with the highest point total following the final round wins the competition. At the end of the competition, several students are awarded outstanding attorney and outstanding witness awards, the top three placing teams are announced.

The following is a list of invitational champions: 2019: York Community High School 2018: St. Charles North High School 2017: St. Charles North High School 2016: Timothy Christian School 2015: Hinsdale Central High School 2014: St. Charles North High School. 2013: Hinsdale Central High School 2012: Hinsdale Central High School 2011: Glenwood High School 2010: Hinsdale Central High School 2009: Hinsdale Central High School 2008: Highland Park High School 2007: Glenbard East High School 2006: Timothy Christian School 2005: Hinsdale Central High School 2004: Timothy Christian School 2003: Timothy Christian School 2002: Timothy Christian School 2001: Boylan Catholic High School 2000: Timothy Christian School 1999: Boylan Catholic High School 1998: Timothy Christian School 1997: Glenbard South High School 1996: Hinsdale Central High School 1995: Mt. Vernon Township High School 1994: Hinsdale Central High School 1993: Glenbard South High School 1992: Glenbard South High School 1991: Hinsdale Central High School 1990: Hinsdale Central High School 1989: St. Charles East High School 1988: Hinsdale Central High School 1987: Marian Central Catholic High School 1986: Glenbard South High School 1985: Glenbard South High School 1984: No winner announced.

1983: No winner announced. The following schools have placed in the top 3 at least once: The winner of the ISBA High School Mock Trial Invitational has represented the state at the National High School Mock Trial Championship since its inaugural tournament in 1984. No Illinois team has won the tournament, although Hinsdale Central High School finished in 2nd place in 2010. Most Illinois finished 13th at the 2019 National Championship in Athens, Georgia