The history of Czechs in Baltimore dates back to the mid-19th century. Thousands of Czechs immigrated to East Baltimore during the late 19th and early 20th centuries, becoming an important component of Baltimore's ethnic and cultural heritage. The Czech community has founded a number of cultural institutions to preserve the city's Czech heritage, including a Roman Catholic church, a heritage association, a gymnastics association, an annual festival, a language school, and a cemetery. During the height of the Czech community in the late 19th century and early 20th century, Baltimore was home to 12,000 to 15,000 people of Czech birth or heritage. The population began to decline during the mid-to-late 20th century, as the community assimilated and aged, while many Czech Americans moved to the suburbs of Baltimore. By the 1980s and early 1990s, the former Czech community in East Baltimore had been almost entirely dispersed, though a few remnants of the city's Czech cultural legacy still remain.

Czech Americans, known in the 19th and early 20th century as Bohemian Americans, are citizens of the United States whose ancestry is wholly or partly originate from the Czech lands, a term which refers to the majority of the traditional lands of the Bohemian Crown, namely Bohemia, Moravia and Czech Silesia. These lands over time have been governed by a variety of states, including the Kingdom of Bohemia, the Austrian Empire, Czechoslovakia, and the Czech Republic also known by its short-form name, Czechia. Germans from the Czech lands who emigrated to the United States are usually identified as German Americans, or, more specifically, as Americans of German Bohemian descent. According to the 2000 U.S. census, there are 1,262,527 Americans of full or partial Czech descent, in addition to 441,403 persons who list their ancestry as Czechoslovak. Historical information about Czechs in America is available thanks to people such as Mila Rechcigl.