Holmes County is a county in the U.S. state of Ohio. As of the 2020 Census, the population was 44,223. Its county seat is Millersburg. The county was formed in 1824 from portions of Coshocton, Tuscarawas and Wayne counties and organized the following year. It was named after Andrew Holmes, an officer killed in the War of 1812.

Millersburg is a village and the county seat of Holmes County, Ohio, United States. Located 66 miles (106 km) south of Cleveland, it is in the heart of Ohio's Amish Country and is part of a large regional tourism industry. The population was 3,151 at the 2020 census. Holmes County Airport, located two miles southwest of Millersburg, serves the county.