Holton is a village and civil parish in South Oxfordshire about 5.5 miles (9 km) east of Oxford. The parish is bounded to the southeast by the River Thame, to the east and north by the Thame's tributary Holton Brook, to the south by London Road and to the west by field boundaries with the parishes of Forest Hill with Shotover and Stanton St John.

The River Thame is a river in Southern England. A tributary of the River Thames, the river runs generally south-westward for about 40 mi (64 km) from its source above the Buckinghamshire town of Aylesbury to the Thames in south-east Oxfordshire.