The Holy Chalice, also known as the Holy Grail, is in Christian tradition the vessel that Jesus used at the Last Supper to share His Blood. The Synoptic Gospels refer to Jesus sharing a cup of wine with the Apostles, saying it was the covenant in his blood. The use of wine and chalice in the Eucharist in Christian churches is based on the Last Supper event. In the late 12th century, the author Robert de Boron associated the pre-existing story of the Holy Grail, a magical item from Arthurian literature, with the Holy Chalice. This association was continued in many subsequent Arthurian works, including the Lancelot-Grail (Vulgate) cycle, the Post-Vulgate Cycle, and Sir Thomas Malory's Le Morte d'Arthur. A cup kept in the Spanish Cathedral of Valencia has been identified since medieval times as the purported Holy Chalice used at the Last Supper.

A chalice or goblet is a footed cup intended to hold a drink. In religious practice, a chalice is often used for drinking during a ceremony or may carry a certain symbolic meaning.