Holy Cross Church is a historic church and home to an active parish in the Roman Catholic Diocese of Columbus located in the Discovery District neighborhood of Columbus, Ohio. The Gothic Revival building was completed in 1848 and is the oldest church in Columbus. The church, along with the school and rectory also on the property, was added to the National Register of Historic Places in 1979. The parish was suppressed and its territory absorbed by St. Joseph Cathedral in 2023, but remains open for public Masses.

The Diocese of Columbus is a Latin Church ecclesiastical territory, or diocese, of the Catholic Church covering 23 counties in central Ohio in the United States. It is a suffragan diocese in the ecclesiastical province of the metropolitan Archdiocese of Cincinnati.