The Holy Lance, also known as the Lance of Longinus, the Spear of Destiny, or the Holy Spear, is alleged to be the lance that pierced the side of Jesus as he hung on the cross during his crucifixion. As with other instruments of the Passion, the lance is only briefly mentioned in the Christian Bible, but later became the subject of extrabiblical traditions in the medieval church. Relics purported to be the lance began to appear as early as the 6th century, originally in Jerusalem. By the Late Middle Ages, relics identified as the spearhead of the Holy Lance had been described throughout Europe. Several of these artifacts are still preserved to this day.

Longinus is the name given to the unnamed Roman soldier who pierced the side of Jesus with a lance; who in medieval and some modern Christian traditions is described as a convert to Christianity. His name first appeared in the apocryphal Gospel of Nicodemus. The lance is called in Christianity the "Holy Lance" (lancea) and the story is related in the Gospel of John during the Crucifixion. This act is said to have created the last of the Five Holy Wounds of Christ.