The Holy of Holies is a term in the Hebrew Bible that refers to the inner sanctuary of the Tabernacle, where the Shekhinah appeared. According to Hebrew tradition, the area was defined by four pillars that held up the veil of the covering, under which the Ark of the Covenant was held above the floor. According to the Hebrew Bible, the Ark contained the Ten Commandments, which were given by God to Moses on Mount Sinai. The first Temple in Jerusalem, called Solomon's Temple, was said to have been built by King Solomon to keep the Ark.

The Ark of the Covenant, also known as the Ark of the Testimony or the Ark of God, is believed to have been the most sacred religious relic of the Israelites. It is described as a wooden chest coated in pure gold and topped off by an elaborate golden lid known as the mercy seat. According to the Book of Exodus and First Book of Kings in the Hebrew Bible and the Old Testament, the Ark contained the Tablets of the Law, by which God delivered the Ten Commandments to Moses at Mount Sinai. According to the Book of Exodus, the Book of Numbers, and the Epistle to the Hebrews in the New Testament, it also contained Aaron's rod and a pot of manna.