The Humboldt Botanical Garden is a 44.5 acres botanical garden located south of Eureka, California, United States. The Garden is near the South Bay portion of Humboldt Bay on the north side of the College of the Redwoods. Grading and site preparation began in August 2003. Featuring views of Humboldt Bay and the Pacific Ocean, the garden opened in 2006, with more development completed by 2008.

Eureka is a city and the county seat of Humboldt County, located on the North Coast of California. The city is located on U.S. Route 101 on the shores of Humboldt Bay, 270 miles (435 km) north of San Francisco and 100 miles (161 km) south of the Oregon border. At the 2020 census, the population of the city was 26,512. As of the 2010 census, the population of Greater Eureka was 45,034.