Crossed immunoelectrophoresis of 2 microlitres of normal human serum. The electrophoresis was performed in thin layers of agarose; the pictured gel is about 7x7 cm. The lower part is the first dimension gel without antibodies, where the serum was applied into the slot at the lower left. The upper part is the second dimension gel with Dako antibodies against human serum proteins. More than 50 major serum proteins can be named.

Fused rocket immunoelectrophoresis of an affinity chromatographic separation of human serum proteins on con A. A 15 microlitre sample from each fraction was applied (starting from left) and allowed to diffuse about one hour, then electrophoresis was performed overnight. Peak "a" was not retained, while peak "b" was retained and eluted with methyl-mannose. The arrow indicates some slightly retained proteins.

Plasmodium Glutamate dehydrogenase (pGluDH) separated by Counterimmunoelectrophoresis

The quantitative principle of affinity electrophoresis illustrated with electrophoresis at pH 8.6 of concanavalin A into an agarose gel containing blood serum (3.6 microliter per square cm). The bar indicates 1 cm. Electrophoresis performed overnight at less than 10 V/cm. The analysis was performed early in the 1970s at the Protein Laboratory