Immunoelectrophoresis
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Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies. All variants of immun
Crossed immunoelectrophoresis of 2 microlitres of normal human serum. The electrophoresis was performed in thin layers of agarose; the pictured gel is about 7x7 cm. The lower part is the first dimension gel without antibodies, where the serum was applied into the slot at the lower left. The upper part is the second dimension gel with Dako antibodies against human serum proteins. More than 50 major serum proteins can be named.
Fused rocket immunoelectrophoresis of an affinity chromatographic separation of human serum proteins on con A. A 15 microlitre sample from each fraction was applied (starting from left) and allowed to diffuse about one hour, then electrophoresis was performed overnight. Peak "a" was not retained, while peak "b" was retained and eluted with methyl-mannose. The arrow indicates some slightly retained proteins.
Plasmodium Glutamate dehydrogenase (pGluDH) separated by Counterimmunoelectrophoresis
Agarose
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Agarose is a polysaccharide, generally extracted from certain red algae. It is a linear polymer made up of the repeating unit of agarobiose, which is a disaccharide made up of D-galactose and 3,6-anhy
Immunoelectrophoresis
…is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies…
An agarose gel in a tray used for gel electrophoresis
An agarose gel with bands of DNA stained with ethidium bromide and visualized under UV light on a UV Transilluminator.
Agarose-based gel filtration columns used for protein purification on an AKTA FPLC machine.