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Immunohistochemistry
Videos
Immunohistochemistry is a form of immunostaining. It involves the process of selectively identifying antigens in cells and tissue, by exploiting the principle of antibodies binding specifically to ant
Main staining patterns on chromogenic immunohistochemistry.
Main staining patterns on chromogenic immunohistochemistry.
Immunofluorescence of human skin using an anti-IgA antibody. The skin is from a patient with Henoch–Schönlein purpura: IgA deposits are found in the w
Immunofluorescence of human skin using an anti-IgA antibody. The skin is from a patient with Henoch–Schönlein purpura: IgA deposits are found in the walls of small superficial capillaries (yellow arrows). The pale wavy green area on top is the epidermis, the bottom fibrous area is the dermis.
"Block" staining: strong nuclear and cytoplasmic expression in a continuous segment of cells.
"Block" staining: strong nuclear and cytoplasmic expression in a continuous segment of cells.
Chromogenic immunohistochemistry: The cell is exposed to a primary antibody (red) that binds to a specific antigen (purple square). The primary antibo
Chromogenic immunohistochemistry: The cell is exposed to a primary antibody (red) that binds to a specific antigen (purple square). The primary antibody binds a secondary (green) antibody that is chemically coupled to an enzyme (blue). The enzyme changes the color of the substrate to a more pigmented one (brown star).
Western blot
Videos
The Western blot, or Western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract, and to vis
Immunohistochemistry
…antibody was produced, the background staining can be reduced. It is also possible to use commercially available universal blocking buffers. Other common blocking buffers include normal serum, non-fat dry milk, BSA, or gelatin. Endogenous enzyme activity may also cause background staining but can be reduced if…
Western blot workflow
Western blot workflow
Western blot HIV test where the first two strips are negative and positive controls followed by actual tests
Western blot HIV test where the first two strips are negative and positive controls followed by actual tests
From gel to publication
From gel to publication