Interstate 110 (I-110) is a 4.1-mile (6.6 km) freeway spur route in Biloxi, Mississippi, running south from I-10 to U.S. Route 90 (US 90). It is one of very few places on the Interstate Highway System utilizing a drawbridge. The southbound control city is Biloxi, with a series of bridges out over the Gulf of Mexico at the southern terminus. There is no northbound control city; the road is marked with trailblazers reading "TO I-10" instead. It ran entirely concurrently with Mississippi Highway 15 (MS 15), until MS 15 was truncated to I-10.

Biloxi is a city in Harrison County, Mississippi, United States. It lies on the Gulf Coast in southern Mississippi, bordering the city of Gulfport to its west. The adjacent cities are both designated as seats of Harrison County. The population of Biloxi was 49,449 at the 2020 census, making it the state's 4th most populous city. It is a principal city of the Gulfport–Biloxi metropolitan area, home to 416,259 residents in 2020. The area's first European settlers were French colonists.