Interstate 35 (I-35) is a north–south Interstate Highway that stretches from Laredo, Texas, to Duluth, Minnesota. In the US state of Minnesota, I-35 enters from Iowa and heads north toward the twin cities of Minneapolis and Saint Paul. South of the metropolitan area, I-35 splits into two branches; I-35E runs through Saint Paul and I-35W through Minneapolis. These two branches rejoin north of the Twin Cities, and the highway continues north to Duluth, where it terminates at State Highway 61 (MN 61). The highway was authorized in 1956 and the first segment opened in 1958. It reached Duluth in 1971, and the final segment to east Duluth opened in 1992.

Interstate 35E (I-35E) is an Interstate Highway in the US state of Minnesota, passing through downtown Saint Paul. It is one of two through routes for I-35 through the Twin Cities of Minneapolis and Saint Paul, the other being I-35W through Minneapolis. Thus, both ends of I-35E are shared with I-35W and I-35.