Interstate 4 (I-4) is an Interstate Highway located entirely within the US state of Florida, maintained by the Florida Department of Transportation (FDOT). Spanning 132.30 miles (212.92 km) along a generally southwest–northeast axis, I-4 is entirely concurrent with State Road 400 (SR 400). In the west, I-4 begins at an interchange with I-275 in Tampa. I-4 intersects with several major expressways as it traverses Central Florida, including US Highway 41 (US 41) in Tampa; US 301 near Riverview; I-75 near Brandon; US 98 in Lakeland; US 27 in unincorporated Davenport; US 192 in Celebration; Florida's Turnpike in Orlando; and US 17 and US 92 in multiple junctions. In the east, I-4 ends at an interchange with I-95 in Daytona Beach, while SR 400 continues for roughly another four miles (6.4 km) and ends at an intersection with US 1 on the city line of Daytona Beach and South Daytona.

U.S. Highway 27 (US 27) in Florida is a north–south United States Numbered Highway. It runs 496.352 miles (798.801 km) from the Miami metropolitan area northwest to the Tallahassee metropolitan area. Throughout the state, US 27 has been designated the Claude Pepper Memorial Highway by the Florida Legislature. It was named after long-time Florida statesperson Claude Pepper, who served in both the U.S. Senate and House of Representatives. The stretch running from Miami to South Bay was originally designated the Thomas E. Will Memorial Highway by the Florida Legislature in 1937 when that portion was known as State Road 26 (SR 26). Thomas E. Will, the founder of Okeelanta, had worked for almost 20 years to get the state to build a road from Miami to the area south of Lake Okeechobee. For most of its length in the state, US 27 is a divided highway.