Interstate 64 (I-64) in the US state of Virginia runs east–west through the middle of the state from West Virginia to the Hampton Roads region, for a total of 299 miles (481 km). From the West Virginia state line to Chesapeake, it passes through the major cities of Lexington, Staunton, Charlottesville, the state capital of Richmond, Newport News, Hampton, and Norfolk. It is notable for crossing the mouth of the harbor of Hampton Roads on the Hampton Roads Bridge–Tunnel (HRBT), the first bridge–tunnel to incorporate artificial islands, concurrent with U.S. Route 60 (US 60). Also noteworthy is a section through Rockfish Gap, a wind gap in the Blue Ridge Mountains, which was equipped with an innovative system of airport-style runway lighting embedded into the pavement to aid motorists during periods of poor visibility due to fog or other conditions.

Newport News is an independent city in Virginia, United States. At the 2020 census, the population was 186,247. Located in the Hampton Roads region, it is the fifth-most populous city in Virginia and 140th-most populous city in the United States.