Interstate 65 (I-65) meanders across 366 miles (589 km) of the Alabama countryside linking six of the state's 10 largest cities. The highway links together many important roadways that make commerce inside and outside of the state's boundaries possible. It starts at I-10 near Mobile. The route passes through the major cities of Montgomery, Birmingham, Decatur, Huntsville, and Athens before entering Tennessee in the north near the town of Ardmore, Alabama.

Decatur is the largest city and county seat of Morgan County in the U.S. state of Alabama. Nicknamed "The River City," it is located in northern Alabama on the banks of Wheeler Lake along the Tennessee River. The population was 57,938 at the 2020 census.