Interstate 95 (I-95) is a part of the Interstate Highway System that runs north–south from Miami, Florida to Houlton, Maine. The highway enters Maine from the New Hampshire state line in Kittery and runs for 303 miles (488 km) to the Canada–United States border at Houlton. It is the only primary Interstate Highway in Maine. In 2004, the highway's route between Portland and Gardiner was changed so that it encompasses the entire Maine Turnpike, a toll road running from Kittery to Augusta. As an Interstate Highway, all of I-95 in Maine is included in the National Highway System, a network of roads important to the country's economy, defense, and mobility.

Houlton is a town and county seat of Aroostook County, Maine, United States, on the Canada–United States border. As of the 2020 census, the town's population was 6,055. It is perhaps best known for being at the northern terminus of Interstate 95 and as the birthplace of Samantha Smith, a goodwill ambassador as a child during the Cold War. The town hosts the annual Houlton Agricultural Fair.