Italy participated in the Junior Eurovision Song Contest 2016 which took place on 20 November 2016, in Valletta, Malta. The Italian broadcaster Rai Gulp, which is a channel owned by Radiotelevisione Italiana (RAI) was responsible for organising their entry for the contest. Fiamma Boccia was internally selected to represent Italy with the song "Cara Mamma ". Italy placed third in the contest with 209 points.

The Junior Eurovision Song Contest 2016 was the fourteenth edition of the annual Junior Eurovision Song Contest, which took place at the Mediterranean Conference Centre, in Valletta, Malta. This was the second time that Malta have hosted the Junior Eurovision Song Contest, their first being in 2014. Jon Ola Sand was appointed as the Executive Supervisor for the 2016 Junior Eurovision Song Contest, following the dismissal of the former supervisor, Vladislav Yakovlev.