Jamestown is a town in Guilford County, North Carolina, United States. It is a suburb of the nearby cities of Greensboro and High Point. The population was 3,382 at the 2010 census.

Guilford County is a county located in the U.S. state of North Carolina. As of the 2020 census, the population was 541,299, making it the third-most populous county in North Carolina. The county seat and largest community is Greensboro. Since 1938, an additional county court has been located in High Point. The county was formed in 1771. Guilford County is included in the Greensboro-High Point, NC Metropolitan Statistical Area, which is also included in the Greensboro–Winston-Salem–High Point, NC Combined Statistical Area.