Jamestown Church, constructed in brick from 1639 onward, in Jamestown in the Mid-Atlantic state of Virginia, is one of the oldest surviving building remnants built by Europeans in the original Thirteen Colonies and in the United States overall. It is now part of Historic Jamestown, and is owned by Preservation Virginia. There have been several sites and stages in the church's history, and its later tower is now the last surviving above-ground structure from the days when Jamestown was the capital of Virginia. The current structure, active as part of the Continuing Anglican movement, is still in use today. The ruins are currently being researched by members of the Jamestown Rediscovery project.

The Jamestown settlement in the Colony of Virginia was the first permanent English settlement in the Americas. It was located on the northeast bank of the James River, about 2.5 mi (4 km) southwest of the center of modern Williamsburg. It was established by the Virginia Company of London as "James Fort" on May 4, 1607 O.S., and was considered permanent after a brief abandonment in 1610. It followed several failed attempts, including the Lost Colony of Roanoke, established in 1585 on Roanoke Island, later part of North Carolina. Jamestown served as the colonial capital from 1616 until 1699. Despite the dispatch of more settlers and supplies, more than 80 percent of the colonists died in 1609–1610, mostly from starvation and disease. In mid-1610, the survivors abandoned Jamestown, though they returned after meeting a resupply convoy in the James River.