Jefferson/USC station is an at-grade light rail station on the E Line of the Los Angeles Metro Rail system. The station is located alongside Flower Street at its intersection with Jefferson Boulevard, after which the station is named, along with the nearby University of Southern California (USC). The station also has nearby stops for the J Line of the Los Angeles Metro Busway system, southbound buses stop on Flower Street, across from the station and northbound buses stop on Figueroa Street, one block to the west. Jefferson/USC station serves the North University Park in neighborhood of Los Angeles.

The E Line is a 22-mile (35 km) light rail line in Los Angeles County, California. It is one of the six lines of the Los Angeles Metro Rail system, operated by the Los Angeles County Metropolitan Transportation Authority (Metro). The E Line runs east-west and serves 29 stations between East Los Angeles and Santa Monica. It interlines and shares five stations with the A Line in Downtown Los Angeles. Service operates for approximately 19 hours with headways of up to 8 minutes during peak hours. The E Line, the second-busiest light rail line in the system, saw an average of 41,902 passengers on weekdays in October 2023.