Jefferson Station is an underground SEPTA Regional Rail station located on Market Street in Philadelphia, Pennsylvania. It is the easternmost of the three Center City stations of the SEPTA Regional Rail system and is part of the Center City Commuter Connection, which connects the former Penn Central commuter lines with the former Reading Company commuter lines. In 2014, the station saw approximately 26,000 passengers every weekday.

The SEPTA Regional Rail system is a commuter rail network owned by SEPTA and serving the Philadelphia metropolitan area. The system has 13 branches and more than 150 active stations in Philadelphia, Pennsylvania, its suburbs and satellite towns and cities. It is the sixth-busiest commuter railroad in the United States, and the busiest outside of the New York, Chicago, and Boston metropolitan areas. In 2016, the Regional Rail system had an average of 132,000 daily riders and 118,800 daily riders as of 2019.