John Astley, also seen as Ashley, was an English courtier, Marian exile, and Master of the Jewel Office. He was a Member of Parliament on many occasions.

Katherine Ashley, also known as Kat Ashley or Astley, was the first close friend, governess, and Lady of the Bedchamber to Queen Elizabeth I of England. She was the aunt of Catherine Champernowne, who was the mother of Sir Humphrey Gilbert from her first marriage and Walter Raleigh by her second marriage.