John Cabot University (JCU) is a private American university in Rome, Italy. It was founded in 1972 and it offers undergraduate degrees, graduate degrees, and study abroad programs to English-speaking students. The university has more than 700 degree-seeking students and more than 1000 visiting students every year, representing over 75 nationalities. The average class size is 15 students. The university consists of three campuses and three residence halls centrally located in Trastevere, Rome. The language of instruction is English.

Trastevere is the 13th rione of Rome, Italy. It is identified by the initials R. XIII and it is located within Municipio I. Its name comes from Latin trans Tiberim.