Johnson Creek is a village in Jefferson County, Wisconsin, United States. The population was 3,318 at the 2020 census. The village is approximately halfway between Milwaukee and Madison, at the intersection of Wisconsin Highway 26 and Interstate 94.

Jefferson County is a county in the U.S. state of Wisconsin. As of the 2020 census, the population was 84,900. Its county seat is Jefferson. Jefferson County comprises the Watertown-Fort Atkinson, WI Micropolitan Statistical Area, which is also included in the Milwaukee-Racine-Waukesha, WI Combined Statistical Area.