The Johor Bahru District is a district located in the southern part of Johor, Malaysia. The heavily suburban/suburbanizing district covers an area of 1,063.97 km2 (410.80 sq mi) and has a population of 1.71 million. The district capital is Johor Bahru City and the administrative capital is Iskandar Puteri City. The district borders Pontian District on the west, Kota Tinggi District on the east, Kulai District on the north and Straits of Johor to the south. The urban centres are divided into the cities of Johor Bahru, Iskandar Puteri and Pasir Gudang.

Johor Bahru, colloquially referred to as JB, is the core city of Johor Bahru District, and the capital city of the state of Johor, Malaysia. It is the second-largest national GDP-contributor among the major cities in Malaysia, and forms a part of Iskandar Malaysia, the nation's largest special economic zone, by investment value. The city has a population of 858,118 people within an area of 391.25 km2.