Tomb KV21 is an ancient Egyptian tomb located in the Valley of the Kings in Egypt. It was discovered in 1817 by Giovanni Belzoni and later re-excavated by Donald P. Ryan in 1989. It contains the mummies of two women, thought to be Eighteenth Dynasty queens. In 2010, a team headed by Zahi Hawass used DNA evidence to tentatively identify one mummy, KV21A, as the biological mother of the two fetuses preserved in the tomb of King Tutankhamun.

The Valley of the Kings, also known as the Valley of the Gates of the Kings, is an area in Egypt where, for a period of nearly 500 years from the Eighteenth Dynasty to the Twentieth Dynasty, rock-cut tombs were excavated for pharaohs and powerful nobles under the New Kingdom of ancient Egypt.