The Karlsruhe–Mühlacker railway is a railway line in the west of the German state of Baden-Württemberg. It was built between 1859 and 1863 and is one of the oldest railways in Germany. It was built as the second connection between the networks of the Grand Duchy of Baden State Railway and the Royal Württemberg State Railways and it still constitutes an important east–west route in southern Germany.

Mühlacker is a town in the eastern part of the Enz district of Baden-Württemberg, Germany. Mühlacker station has direct rail connections with Stuttgart, Karlsruhe, Heidelberg, Pforzheim and the Northern Black Forest.