Kazakhstan competed at the 2016 Summer Olympics in Rio de Janeiro, Brazil, from 5 to 21 August 2016. It was the nation's sixth consecutive appearance at the Summer Olympics in the post-Soviet era. The National Olympic Committee of the Republic of Kazakhstan fielded a roster of 104 athletes, 55 men and 49 women, to compete across seventeen different sports at these Games, the smallest Summer Olympic team since the nation's debut in Atlanta 1996. Moreover, Kazakhstan did not send teams in any of the team sports for the first time in twenty years. Track and field accounted for the largest number of athletes on the Kazakh squad, with 25 entries. There was a single competitor each in slalom canoeing, track cycling, fencing, and table tennis.

During the Parade of Nations within the Rio de Janeiro 2016 Summer Olympics opening ceremony, athletes and officials from each participating team marched in the Maracanã Stadium preceded by their flag and placard bearer. Each flag bearer had been chosen either by the team's National Olympic Committee or by the athletes themselves.