The 2016 24 Hours of Daytona was an International Motor Sports Association (IMSA)-sanctioned 24-hour automobile endurance race for Prototype and Grand Touring sports cars held at the Daytona International Speedway combined road course in Daytona Beach, Florida, from January 30 to 31, 2016. It was the first of twelve 2016 IMSA SportsCar Championship races, the 54th 24 Hours of Daytona, and the first of the four-round North American Endurance Cup. The event was attended by approximately 35,000 people.