Labis is a town and a mukim (township) in Segamat District in northern Johor, Malaysia. A main trunk road that runs north-south Peninsular Malaysia passes through it, as well as the KTM railway line that connects it with the state's capital in the south, Johor Bahru.

The Segamat District or simply Segamat is a district in the Malaysian state of Johor. Segamat is also the name of the district's primary town. Segamat district is one of the three landlocked districts in Johor, the other being Kluang and Kulai.