In the Hebrew Bible, the Levitical cities were 48 cities in ancient Israel set aside for the tribe of Levi, who were not allocated their own territorial land when the Israelites entered the Promised Land.

According to the Bible, the Tribe of Levi is one of the tribes of Israel, traditionally descended from Levi, son of Jacob. The descendants of Aaron, who was the first High Priest of Israel, were designated as the priestly class, the Kohanim.