Lexington is a city in and the county seat of Holmes County, Mississippi, United States. The county was organized in 1833 and the city in 1836. The population was 1,731 at the 2010 census, down from 2,025 at the 2000 census. The estimated population in 2018 was 1,496. It has declined from its high of 3,198 in 1950 due to the expansion of industrial-scale agriculture.

Holmes County is a county in the U.S. state of Mississippi; its western border is formed by the Yazoo River and the eastern border by the Big Black River. The western part of the county is within the Yazoo-Mississippi Delta. As of the 2020 census, the population was 17,000. Its county seat is Lexington. The county is named in honor of David Holmes, territorial governor and the first governor of the state of Mississippi and later United States Senator for Mississippi. Holmes County native, Edmond Favor Noel, was an attorney and state politician, elected as governor of Mississippi, serving from 1908 to 1912.