Little St Mary's or St Mary the Less is a Church of England parish church in Cambridge, England, on Trumpington Street between Pembroke College's Mill Lane Project development site and Peterhouse. The church is in the Diocese of Ely and follows the Anglo-Catholic or high-church tradition of the Church of England. In addition to its main Sunday Mass, the church has a strong tradition of daily morning and evening prayer, regular weekday Communion and the keeping of church festivals. The church has a particular ministry helping both men and women to explore possible vocations to the priesthood. Little St Mary's has active overseas mission links, provides support to local mental health projects, and participates in Hope Cambridge's Churches Homeless Project. The vicar is Canon Robert Mackley, who has been the incumbent since 2012.

Charles Eamer Kempe was a British Victorian era designer and manufacturer of stained glass. His studios produced over 4,000 windows and also designs for altars and altar frontals, furniture and furnishings, lychgates and memorials that helped to define a later nineteenth-century Anglican style. The list of English cathedrals containing examples of his work includes: Chester, Gloucester, Hereford, Lichfield, Wells, Winchester and York. Kempe's networks of patrons and influence stretched from the Royal Family and the Church of England hierarchy to the literary and artistic beau monde.