Live Oak is a city and the county seat of Suwannee County, Florida, United States. The city is located at the midpoint between Tallahassee and Jacksonville. As of 2020, the population recorded by the U.S. Census Bureau was 6,735.

Suwannee County is a county located in the north central portion of the state of Florida. As of the 2020 census, the population was 43,474, up from 41,551 in 2010. Its county seat is Live Oak. Suwannee County was a dry county until August 2011, when the sale of alcoholic beverages became legal in the county.