London Buses route 16 is a Transport for London contracted bus route in London, England. Running between Brent Park and Paddington station, it is operated by Metroline.

Paddington, also known as London Paddington, is a London railway station and London Underground station complex, located on Praed Street in the Paddington area. The site has been the London terminus of services provided by the Great Western Railway and its successors since 1838. Much of the main line station dates from 1854 and was designed by Isambard Kingdom Brunel. As of the 2022–23 Office of Rail & Road Statistics, it is the second busiest station in the United Kingdom, after London Liverpool Street, with 59.2 million entries and exits.