Diesel locomotives have seen limited use on the London Underground, largely because exhaust gases cannot be discharged when the vehicles are working in tunnels. A prototype diesel engine numbered DEL120 was built in 1939 from two 1915 stock motor cars, which was expected to be part of a batch of ten, but experience with battery locomotives showed that these were a better alternative. Three 0-6-0 diesels (DL81-DL83) were obtained in 1971, to replace the last steam engines, but were too short to operate the signalling system, and too heavy for some of the bridges. In 1996, fourteen diesels were supplied by Schöma of Germany, which were used during the construction of the Jubilee line tunnels. They were fitted with exhaust scrubbers, to enable them to work in the tunnels. To speed up track renewals on the subsurface lines, Class 66 locomotives have been hired in since 2006 to handle permanent way trains, but again suffer from being too heavy for some of the bridges. Because they are not fitted with tripcock safety devices, and pull trains much longer than the signalling system is designed for, they are restricted to slow speed running.

London Underground battery-electric locomotives are battery locomotives used for hauling engineers' trains on the London Underground network where they can operate when the electric traction current is switched off. The first two locomotives were built in 1905 for the construction of the Great Northern, Piccadilly and Brompton Railway, and their success prompted the District Railway to buy two more in 1909, which were the only ones built to the loading gauge of the subsurface lines. Following this, a number of battery vehicles were built by converting redundant motor cars, with the batteries placed in the unused passenger compartment. One exception to this was made by the City and South London Railway, who used a trailer car to hold the batteries, and wired them to a separate locomotive.