M-65 is a 103.176-mile-long (166.046 km) state trunkline highway in the US state of Michigan. The highway runs between termini on US Highway 23 (US 23) near Omer and Rogers City in the northeastern Lower Peninsula of the state. M-65 runs inland through several small communities in the region, passing through forests and fields along its course. M-65 crosses several watercourses, including the Au Sable River where it runs along the River Road National Scenic Byway. The region also includes the Huron National Forest and the Mackinaw State Forest areas.

US Highway 23 (US 23) is a north–south United States Numbered Highway that runs from Jacksonville, Florida, to Mackinaw City, Michigan. In the US state of Michigan, it is a major, 362-mile-long (583 km), north–south state trunkline highway that runs through the Lower Peninsula. The trunkline is a freeway from the Michigan–Ohio state line near Lambertville to the city of Standish, and it follows the Lake Huron shoreline from there to its northern terminus. Serving the cities of Ann Arbor and Flint, US 23 acts as a freeway bypass of the Metro Detroit area. Overall, the highway runs through rural areas of the state dominated by farm fields or woodlands; some segments are urban in character in the Ann Arbor, Flint and Tri-Cities areas. The section from Flint north to Standish also carries Interstate 75 (I-75) along a concurrency that includes a segment that carries almost 70,000 vehicles on a daily basis.