The Madison Avenue Line is a line of the Memphis Area Transit Authority trolley system. The trolley line began operating in 2004, and cost $56 million to build. It consists of 2.2 mi (3.5 km) of double track along Madison Avenue with six stops stretching into Midtown Memphis. The line was built to connect the Main Street system to the Medical District just east of Downtown Memphis. Trolley service has been suspended since April 2014, after two trolley cars caught fire within a span of six months. Buses have been serving the line since then, but MATA plans to restore trolley operation to the line eventually.

The Memphis Area Transit Authority (MATA) is the public transportation provider for Memphis, Tennessee. It is one of the largest transit providers in the state of Tennessee; MATA transports customers in the City of Memphis and parts of Shelby County on fixed-route buses, paratransit vehicles, demand-responsive service, and the MATA Trolley system. The system is managed by a seven-member policy board appointed by the mayor and approved by the Memphis City Council. In 2023, the system had a ridership of 3,122,700.