The Mafia Commission Trial was a criminal trial before the United States District Court for the Southern District of New York in New York City, United States, that lasted from February 25, 1985, until November 19, 1986. Using evidence obtained by the Federal Bureau of Investigation, 11 organized crime figures, including the heads of New York City's "Five Families", were indicted by United States Attorney Rudolph Giuliani under the Racketeer Influenced and Corrupt Organizations Act (RICO) on charges including extortion, labor racketeering, and murder. Eight of them were convicted under RICO, and most were sentenced to 100 years in prison on January 13, 1987, the maximum possible sentence under that law.

The Commission is the governing body of the American Mafia, formed in 1931 by Charles "Lucky" Luciano following the Castellammarese War. The Commission replaced the title of capo di tutti i capi, held by Salvatore Maranzano before his murder, with a ruling committee that consists of the bosses of the Five Families of New York City, as well as the bosses of the Chicago Outfit and, at various times, the leaders of smaller families, such as Buffalo, Philadelphia, Detroit, and others. The purpose of the Commission was to oversee all Mafia activities in the United States and serve to mediate conflicts among families.