Magdalena de Kino is a city, part of the surrounding municipality of the same name, located in the Mexican state of Sonora covering approximately 560 square miles. According to the 2005 census, the city's population was 23,101, and the municipality's population was 25,500. Magdalena de Kino is in the northern section of Sonora 50 miles from the Mexico-U.S. border. To the north the municipality abuts Nogales; to the south, the municipality of Santa Ana; to the east, Ímuris and Cucurpe; and to the west, the municipalities of Tubutama and Sáric. Its main sectors include San Ignacio, San Isidro, Tacicuri, and Sásabe. The city was named after the pioneer Roman Catholic missionary and explorer, Father Eusebio Francisco Kino, who worked in the area, as well as in the present-day US state of Arizona.

Sonora, officially Estado Libre y Soberano de Sonora, is one of the 31 states which, along with Mexico City, comprise the Federal Entities of Mexico. The state is divided into 72 municipalities; the capital city of which being Hermosillo, located in the center of the state. Other large cities include Ciudad Obregón, Nogales, San Luis Río Colorado, and Navojoa.