Magnolia Plantation and Gardens is a historic house with gardens located on the Ashley River at 3550 Ashley River Road west of Ashley, Charleston County, South Carolina. It is one of the oldest plantations in the South, and listed on the National Register of Historic Places. Magnolia Plantation is located near Charleston and directly across the Ashley River from North Charleston. The house and gardens are open daily; an admission fee is charged.

The Ashley River is a blackwater and tidal river in South Carolina, rising from the Wassamassaw and Great Cypress Swamps in western Berkeley County. It consolidates its main channel about five miles west of Summerville, widening into a tidal estuary just south of Fort Dorchester. The river then flows for approximately 17 mi (27 km) along the historical banks of the City of North Charleston before reaching peninsular Charleston. The much wider Ashley joins the Cooper River off the Battery in Charleston to form Charleston Harbor before discharging into the Atlantic Ocean. As of 2012 the land around the Ashley River is mostly undeveloped.