Margaret Fleming, Countess of Atholl (1536-1586) was a Scottish courtier and landowner rumoured to be involved in the occult. She served as lady-in-waiting to Mary, Queen of Scots.

Janet Stewart, Lady Fleming, called la Belle Écossaise, was a Scottish courtier. She was an illegitimate daughter of King James IV of Scotland who served as governess to her half-niece Mary, Queen of Scots. Janet was briefly a mistress of King Henry II of France, by whom she had a legitimated son: Henri d'Angoulême. Her daughter, Mary Fleming, was one of the young queen's "Four Marys".