Margaret Tudor was Queen of Scotland from 1503 until 1513 by marriage to King James IV. She then served as regent of Scotland during her son's minority, and fought to extend her regency. Margaret was the eldest daughter and second child of King Henry VII of England and Elizabeth of York, and the elder sister of King Henry VIII of England. By her line, the House of Stuart eventually acceded to the throne of England, in addition to Scotland.

James IV was King of Scotland from 11 June 1488 until his death at the Battle of Flodden in 1513. He inherited the throne at the age of fifteen on the death of his father, James III, at the Battle of Sauchieburn, following a rebellion in which the younger James was the figurehead of the rebels. James IV is generally regarded as the most successful of the Stewart monarchs. He was responsible for a major expansion of the Scottish royal navy, which included the founding of two royal dockyards and the acquisition or construction of 38 ships, including the Michael, the largest warship of its time.